Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
United States Biological Corporation Tris Base Ultrapure, 77-86-1, MFCD00004679, 5kg
Molecular Formula C₄H₁₁NO₃, Purity ≥99.9%, Molecular Biology Grade, Molecular Weight 121.14, Tris is one of the most widely used buffers in molecular biology and cell culture due to its low toxicity, stability and buffering capacity.
Electron Microscopy Sciences Phosphate Buffered Saline, 10X 1 GL
PBS is commonly used in biochemistry. It is a salty solution containing sodium chloride, potassium chloride, sodium phosphate and potassium phosphate. PBS is isotonic and non-toxic to cells. 1X PBS final concentration is 8.0 g/L NaCl, 0.2 g/L KCl, 1.42 g/L Na2HPO4, 0.24 g/L KH2PO4 and pH 7.4.
Medchemexpress LLC 2-Methylthioadenosine diphosphate trisodium | 475193-31-8 | 99.9% | 539.24 | 1 MG
2-Methylthioadenosine diphosphate trisodium is a potent purinergic P2Y receptors agonist. It induces platelet aggregation and shape change, and inhibits cyclic AMP accumulation in platelets exposed to prostaglandin E1.
- Potent purinergic P2Y receptors agonist
- Induces platelet aggregation and shape change
- Inhibits cyclic AMP accumulation
Medchemexpress LLC 2-Morpholinoethanesulphonic acid monohydrate | 145224-94-8 | 99.9% | 500 G
2-Morpholinoethanesulphonic acid monohydrate | 145224-94-8 | 99.9% | 500 G
Gold Biotechnology Inc MES Free Acid 100 g
MES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al It is a zwitterionic morpholinic buffer that is useful for a pH range of 5 5 6 7 and commonly used for cell culture media as a running buffer in electrophoresis and for protein purification in chromatography MES lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions MES is often used in buffered culture media for bacteria yeast and mammalian cells It is toxic towards plants at high concentrations but can be used in culture media at lower concentrations for plants Due to low ionic mobility MES is regarded as an excellent buffer for use in capillary electrochromatography MES is often employed as a substitute for the highly toxic cacodylate as well as a substitute for the non-zwitterionic buffers citrate and malate
Biocare Medical, LLC Tris Buffer Saline (TBS) Plus, 10X
For laboratory use and procedures requiring a high quality Tris buffer saline. TBS is sodium azide and thimerosal free and provides superior pH stability. It can also be used for manual immunohistochemistry applications or on an automated staining system with the addition of surfactant.
Boston Bioproducts Inc MES Buffer (1 M, pH 5.0) - 250ml
MES Buffer [2- (N-morpholino) ethanesulfonic acid] is a zwitterionic Good's buffer used in biological and biochemical research. It maintains a stable pH within the range of 5.5 to 7.0 and is highly soluble in water. MES is commonly used as a running buffer for bis-tris gels, chromatography, and in protein purification. It is valuable for enzymatic reactions, and acts as a non-coordinating buffer in metal ion chemistry. It has minimal UV absorbance and is stable in various applications.
